Input: Raw RNAseq files (.fastq.gz)
Web-based processing (this site)
Local processing (highly recommended)
- Unlimited data size
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Install the
EOA local version in a docker and process your data locally.
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Brief instructions here
Species with good reference transcriptomes
Ultra-fast pseudo-alignment based on Kallisto algorithm
Species without reference transcriptomes
Ultra-fast translated alignment using
Seq2Fun algorithm
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Comprehensive coverage across
~30 taxonomy groups
- Gene-level annotation based on ortholog searching
Output
- Gene/transcript quantification table;
- Gene/transcript annotation table;
- Best hits
- KEGG orthologs (KOs)
- Gene ontologies (GOs)
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Visualization of functional profiles
Upload the result tables to ExpressAnalyst
for in-depth statistical and functional analysis